Journal: Molecular Cell

Article Title: Ribonucleotide Reductase Requires Subunit Switching in Hypoxia to Maintain DNA Replication

doi: 10.1016/j.molcel.2017.03.005

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-53BP1 , Novus Biologicals , Cat# NB100-904.

Techniques: Transduction, Recombinant, Protease Inhibitor, SYBR Green Assay, Mutagenesis, Purification, Gel Extraction, Imaging, Sequencing, Negative Control, Real-time Polymerase Chain Reaction, Software, Expressing

Journal: Aging Cell

Article Title: Superoxide Activates Ferroptosis via the Haber‐Weiss Reaction and Enhances Age‐Related Macular Degeneration

doi: 10.1111/acel.70195

Figure Lengend Snippet: Ferroptotic damage in rental tissues of Sod2 +/− mice exposed to blue light. (A) H&E pathological images of phototoxicity in mice retinal tissues. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer, and RPE layer. (B) Quantification of the thickness of ONL layers in mice rental tissues ( n = 5). (C) Fluorescent imaging RPE layer cell integrity using a ZO‐1 fluorescent antibody. (D) Quantification of RPE cell numbers in the tissues ( n = 5). (E) Colocalization of 4‐HNE and MnSOD in the tissues with DAPI nuclear staining. (F, G) Quantification of the intensity of 4‐HNE and the content of MDA in the tissues ( n = 5). (H) Colocalization of GPX4 and MnSOD with DAPI nuclear staining in the tissues. (I, J) Quantification of MnSOD and GPX4 images in the tissues ( n = 5). (K, L) Quantification of MnSOD and GPX4 activities in the tissues ( n = 5). The results are presented as the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, no significance ( p > 0.05), two‐way ANOVA. Scale bars: 50 μm in (A, E, H), 20 μm in (C).

Article Snippet: Human RPE cell line (ARPE‐19) and human lens epithelium (HLE) were obtained from the American Type Culture Collection (ATCC, USA).

Techniques: Imaging, Staining

Journal: Aging Cell

Article Title: Superoxide Activates Ferroptosis via the Haber‐Weiss Reaction and Enhances Age‐Related Macular Degeneration

doi: 10.1111/acel.70195

Figure Lengend Snippet: Suppression of RPE cell ferroptosis by overexpressing GPX4. (A) Immunoblots of MnSOD and GPX4 in GPX4‐overexpressed RPE cells. (B) The relative GPX4 activities in the cells ( n = 4). (C) After exposure to blue light, the MDA levels in the cells ( n = 4). (D) After exposure to blue light, fluorescent image of oxidized and reduced lipids in the cells. (E) The ratio of oxidized lipids to reduced lipids in the cells ( n = 4). (F) Fluorescent image of 4‐HNE and ferrous ions in the cells. (G, H) Flow cytometry quantifying 4‐HNE and ferrous ions ( n = 4). (I) GPX4 protective effect of RPE cell survival against phototoxicity ( n = 4). The results are presented as the mean ± SD. ** p < 0.01, *** p < 0.001, **** p < 0.0001, two‐way ANOVA. Scale bars: 40 μm (D, F).

Article Snippet: Human RPE cell line (ARPE‐19) and human lens epithelium (HLE) were obtained from the American Type Culture Collection (ATCC, USA).

Techniques: Western Blot, Flow Cytometry

Journal: Aging Cell

Article Title: Superoxide Activates Ferroptosis via the Haber‐Weiss Reaction and Enhances Age‐Related Macular Degeneration

doi: 10.1111/acel.70195

Figure Lengend Snippet: The effect of GPX4 on RPE cell mitochondrial function maintenance. (A) OCR analysis of GPX4‐overexpressed cells exposed to blue light. (B) Quantification of OCR at four different stages ( n = 3). (C) Fluorescent images of JC‐1 aggregate form (red fluorescence) and monomer form (green fluorescence). Flow cytometry quantifying the JC‐1 aggregate and monomer forms. (D), Quantitative JC‐1 aggregate fluorescent intensity ( n = 3). (E), Flow cytometry quantifying the JC‐1 monomer form ( n = 3). (F) EM photograph of mitochondrial structures in the cells. The results are presented as the mean ± SD. ** p < 0.01**** p < 0.0001, comparisons in same groups. #### p < 0.0001, comparisons in different groups, two‐way ANOVA. Scale bars: 40 μm in (C); 1 μm in (F) top panels, and 100 nm in (F) low panel.

Article Snippet: Human RPE cell line (ARPE‐19) and human lens epithelium (HLE) were obtained from the American Type Culture Collection (ATCC, USA).

Techniques: Fluorescence, Flow Cytometry